FLIMfit v5.0.3

We are pleased to announce the release of version 5.0.3 of the open-source Fluorescence Lifetime fitting and visualisation tool FLIMfit, providing a number of new features and performance enhancements. Download version 5.0.3 here.

A guide to using FLIMfit for analysing multiphoton FLIM data with tutorial videos is available in a recent paper, Conway, JRW, Warren, SC & Timpson, P, Context-dependent intravital imaging of therapeutic response using intramolecular FRET biosensors, Methods (2017).

New features

  • New segmentation manager with new freehand tool options and improved performance
  • Estimate IRF from a mono-exponential reference using an exponentially modified gaussian model. This can produce a high quality IRF for TCSPC detectors without secondary peaks or significant afterpulsing
  • Add support for HydraHarp and TimeHarp data files
  • Add support for data acquired during bidirectional scan
  • Where possible determine repetition rate from data (currently ptu/pt3 files)

Performance Enhancements

  • Significantly improved loading times for some data formats
  • Added option to display smoothed data in the decay tab. When off, this significantly reduces the time to switch between datasets. This option does not affect smoothing during data fitting.
  • Improved performance selecting/deselecting images to fit
  • Update to Bioformats 5.4

Other Enhancements

  • Add option to plot weighted or unweighted values in plotter
  • Visual enhacements to plotter
  • Enable plotting by image number
  • Allow setting default Gate Max value
  • Improved support for older MacOS X versions (10.9.x)

Bug fixes

  • Allow t0 shift map estimation from any loaded dataset (rather than first only)
  • Fix crash when fitting t0 and offset locally

FLIMfit v4.12.1

We are pleased to announce the release of version 4.12.1 of the open-source Fluorescence Lifetime fitting and visualisation tool FLIMfit. Download version 4.12.1 here.

Improvements

  • Reenabled support for constraining global fitting scope as specified by a variable
  • Delay clearing data until loading new data is complete
  • Signed windows installer to prevent smart screen warning on download

Bug fixes

  • Fixed issue with cross platform segmentation manager display
  • Fixed issue loading 1D line scans from .bin files
  • Fixed issue loading/saving IRF shift maps causing failures in segmentation manager

Help pages can be found at: http://help.openmicroscopy.org/flimfit.html

The users list for this software can be found at: http://lists.openmicroscopy.org.uk/mailman/listinfo/flimfit-users

FLIMfit v4.12.0

We are pleased to announce the release of version 4.12.0 of the open-source Fluorescence Lifetime fitting and visualisation tool FLIMfit. Download version 4.12.0 here.

Updates/Improvements

  • Added support for Windows 10

  • This version is built with Bio-Formats 5.2 (specifically version 5.2.2) which, among other improvements, includes support for Becker & Hickl .spc FIFO data and performance improvements for loading large .sdt FLIM files.

Full details of all of the Bio-Formats changes are available on the Bio-Formats version history page - http://www.openmicroscopy.org/site/support/bio-formats5.2/about/whats-new.html

Help pages can be found at: http://help.openmicroscopy.org/flimfit.html

The users list for this software can be found at: http://lists.openmicroscopy.org.uk/mailman/listinfo/flimfit-users

FLIMfit v4.11.2

We are pleased to announce the release of version 4.11.1 of the open-source Fluorescence Lifetime fitting and visualisation tool FLIMfit. Download version 4.11.2 here.

Updates/Improvements

  • FLIMfit is now capable of handling some multi-dimensional file-sets. For example, if you have a real-time time-series of z-stacks, with one z-stack in each of several files, it can now be successfully loaded and processed.
  • The “Files->Load FLIM Data” menu item now allows the user to select multiple files.
  • The “Files->Load FLIM Dataset” menu item has been renamed, for clarity, to “Load FLIM Data from a Directory”.
  • The maximum number of segmented regions has been increased to 4095.
  • Another waitbar has been added to make the workflow clearer when using large datasets.
  • A method for estimating a Gaussian IRF from single-exponential reference data has been added
  • Loading Picoquant files created on MT200 systems is now supported

Bug Fixes

  • An issue with loading polarisation resolved IRFs, with different time points to the data, has been addressed
  • An issue with the loading of time-varying backgrounds has been addressed.
  • An issue loading time gated data with legacy file names has been addressed

Downloads and documentation for FLIMfit are available at http://flimfit.org

Help pages can be found at: http://help.openmicroscopy.org/flimfit.html

The users list for this software can be found at: http://lists.openmicroscopy.org.uk/mailman/listinfo/flimfit-users

FLIMfit v4.11.1

We are pleased to announce the release of version 4.11.1 of the open-source Fluorescence Lifetime fitting and visualisation tool FLIMfit. Download version 4.11.1 here.

Bug Fixes

  • A problem with the FLIMfit Windows installer has been resolved. A third-party dependency downloaded by the installer was moved, causing the downloader to fail.