11 Jul 2018
We are pleased to announce the release of version 5.1.1 of the open-source Fluorescence Lifetime fitting and visualisation tool FLIMfit, providing a number of new features and performance enhancements. Download version 5.1.1 here.
Please note that this version of FLIMfit uses Maximum Likelihood fitting by default. This is the best choice for TCSPC datasets..
A guide to using FLIMfit for analysing multiphoton FLIM data with tutorial videos is available in a recent paper, Conway, JRW, Warren, SC & Timpson, P, Context-dependent intravital imaging of therapeutic response using intramolecular FRET biosensors, Methods (2017).
New Features
- New phasor-plot based segmentation allows, for example, gating out of tissue autofluorescence
- Load motion-corrected FLIM data from Galene
Enhancements
- Use Maximum Likelihood fitting by default
- Improved IRF estimation using photodiode model
- Load compressed B&H FIFO data with updated Bioformats
- Improve compatibility with LaVision .ome.tifs
- Save colourscale limits in exported image metadata
Bug fixes
- Fix ability to load plain TIF stacks
- Fix regression in reference reconvolution
flimfit-users@lists.openmicroscopy.org.uk
06 Jun 2017
We are pleased to announce the release of version 5.0.3 of the open-source Fluorescence Lifetime fitting and visualisation tool FLIMfit, providing a number of new features and performance enhancements. Download version 5.0.3 here.
A guide to using FLIMfit for analysing multiphoton FLIM data with tutorial videos is available in a recent paper, Conway, JRW, Warren, SC & Timpson, P, Context-dependent intravital imaging of therapeutic response using intramolecular FRET biosensors, Methods (2017).
New features
- New segmentation manager with new freehand tool options and improved performance
- Estimate IRF from a mono-exponential reference using an exponentially modified gaussian model.
This can produce a high quality IRF for TCSPC detectors without secondary peaks or significant afterpulsing
- Add support for HydraHarp and TimeHarp data files
- Add support for data acquired during bidirectional scan
- Where possible determine repetition rate from data (currently ptu/pt3 files)
- Significantly improved loading times for some data formats
- Added option to display smoothed data in the decay tab. When off, this significantly reduces the time to switch between datasets.
This option does not affect smoothing during data fitting.
- Improved performance selecting/deselecting images to fit
- Update to Bioformats 5.4
Other Enhancements
- Add option to plot weighted or unweighted values in plotter
- Visual enhacements to plotter
- Enable plotting by image number
- Allow setting default Gate Max value
- Improved support for older MacOS X versions (10.9.x)
Bug fixes
- Allow t0 shift map estimation from any loaded dataset (rather than first only)
- Fix crash when fitting t0 and offset locally
29 Nov 2016
We are pleased to announce the release of version 4.12.1 of the open-source Fluorescence Lifetime fitting and visualisation tool FLIMfit. Download version 4.12.1 here.
Improvements
- Reenabled support for constraining global fitting scope as specified by a variable
- Delay clearing data until loading new data is complete
- Signed windows installer to prevent smart screen warning on download
Bug fixes
- Fixed issue with cross platform segmentation manager display
- Fixed issue loading 1D line scans from .bin files
- Fixed issue loading/saving IRF shift maps causing failures in segmentation manager
Help pages can be found at: http://help.openmicroscopy.org/flimfit.html
The users list for this software can be found at: http://lists.openmicroscopy.org.uk/mailman/listinfo/flimfit-users
21 Sep 2016
We are pleased to announce the release of version 4.12.0 of the open-source Fluorescence Lifetime fitting and visualisation tool FLIMfit. Download version 4.12.0 here.
Updates/Improvements
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Added support for Windows 10
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This version is built with Bio-Formats 5.2 (specifically version 5.2.2) which, among other improvements, includes support for Becker & Hickl .spc FIFO data and performance improvements for loading large .sdt FLIM files.
Full details of all of the Bio-Formats changes are available on the Bio-Formats version history page -
http://www.openmicroscopy.org/site/support/bio-formats5.2/about/whats-new.html
Help pages can be found at: http://help.openmicroscopy.org/flimfit.html
The users list for this software can be found at: http://lists.openmicroscopy.org.uk/mailman/listinfo/flimfit-users
24 Aug 2016
We are pleased to announce the release of version 4.11.1 of the open-source Fluorescence Lifetime fitting and visualisation tool FLIMfit. Download version 4.11.2 here.
Updates/Improvements
- FLIMfit is now capable of handling some multi-dimensional file-sets. For example, if you have a real-time time-series of z-stacks, with one z-stack in each of several files, it can now be successfully loaded and processed.
- The “Files->Load FLIM Data” menu item now allows the user to select multiple files.
- The “Files->Load FLIM Dataset” menu item has been renamed, for clarity, to “Load FLIM Data from a Directory”.
- The maximum number of segmented regions has been increased to 4095.
- Another waitbar has been added to make the workflow clearer when using large datasets.
- A method for estimating a Gaussian IRF from single-exponential reference data has been added
- Loading Picoquant files created on MT200 systems is now supported
Bug Fixes
- An issue with loading polarisation resolved IRFs, with different time points to the data, has been addressed
- An issue with the loading of time-varying backgrounds has been addressed.
- An issue loading time gated data with legacy file names has been addressed
Downloads and documentation for FLIMfit are available at http://flimfit.org
Help pages can be found at: http://help.openmicroscopy.org/flimfit.html
The users list for this software can be found at: http://lists.openmicroscopy.org.uk/mailman/listinfo/flimfit-users
